Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Herpes Simplex Virus Type 1 Infects Enteric Neurons and Triggers Gut Dysfunction via Macrophage Recruitment

doi: 10.3389/fcimb.2018.00074

Figure Lengend Snippet: Primary and secondary antibodies used in the study.

Article Snippet: CD8 (rabbit) , orb1269 , Biorbyt Ltd , FC.

Techniques:

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Herpes Simplex Virus Type 1 Infects Enteric Neurons and Triggers Gut Dysfunction via Macrophage Recruitment

doi: 10.3389/fcimb.2018.00074

Figure Lengend Snippet: Weak adaptive immune response in the LMMP following HSV-1 administration. (A) Sections of ileum obtained from sham or HSV-1 strain SC16 infected mice were subjected to immunohistochemistry for CD3. Scale bars: 40 μm. Representative images of three separate experiments. (B) Freshly collected LMMP were digested and the resulting cell suspension were labeled with anti-CD3 antibody and analyzed by flow cytometry. CD3 + cells were expressed as percentage of 10 5 collected events. (C) Cell suspensions obtained from LMMP as previously described were labeled with anti-CD3, anti-CD4 or anti-CD8 antibodies and analyzed by flow cytometry. CD4:CD8 ratio of 1 × 10 5 CD3 + cells was calculated. (D,E) Cell suspensions obtained from LMMP were incubated for 16 h in presence or absence of UV-inactivated HSV-1 strain SC16. Cells were then collected, labeled with anti-CD3, anti-CD8 and anti-IFNγ or anti-CD3, anti-CD4 and anti-IL4 antibodies and analyzed by flow cytometry in 5 × 10 4 collected events. Representative images of at least five separate experiments are reported. (F,G) Percentages of fluorescence as reported in (D,E) were graphed. N = 5 mice per group. °Denotes P < 0.05 compared to no HSV-1 pulsed cells at the same time point. (H) Mice infected with HSV-1 strain 16 were intraperitoneally injected with rat anti-mouse CD4 purified monoclonal antibody (anti-CD4 Ab). Two weeks post-IG infection, mice received IG non-absorbable FITC-labeled dextran. Sixty minutes later mice were sacrificed. Gastric emptying was calculated as the percentage of probe retained into the stomach compared with the total amount of fluorescence in the gastrointestinal tract. (I) Distribution of FITC-labeled dextran was determined in the intestine. Intestinal transit was reported as the geometric center of distribution of the fluorescent probe throughout the ileum. (J) Time (seconds, sec) required for expulsion of a glass bead inserted into the rectum. Data are represented as mean ± SEM. n = 6–9 mice per group. * Denotes P < 0.05 compared to sham infected mice.

Article Snippet: CD8 (rabbit) , orb1269 , Biorbyt Ltd , FC.

Techniques: Infection, Immunohistochemistry, Labeling, Flow Cytometry, Incubation, Fluorescence, Injection, Purification

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Oncolytic adenovirus in treating malignant ascites: A phase II trial and longitudinal single-cell study.

doi: 10.1016/j.ymthe.2024.04.029

Figure Lengend Snippet: Figure 4. Characteristics and dynamics of CD8+ and CD4+ T cell subsets during H101 treatment (A) Distinct cell subtypes of CD8+ T cells demonstrated by UMAP plot. (B) Cell proportion of each CD8+ T cell subtype. Black asterisks represent the statistical significantly increased proportion on day 7 compared with that at baseline. (C) Fraction of CD8_MKI67 cells in two response groups on days 0, 7, and 14. Wilcoxon test. *p < 0.05. (D) Representative double-color immunofluorescence staining of CD8+Ki67+ cells in MA samples (P04 [T-long patient] and P07 [T-short patient]) on days 0, 7, and 14, with

Article Snippet: For double-color immunofluorescence of CD8A and Ki67, mouse anti-human CD8a (CST, Cat#70306) and rabbit anti-human Ki67 (Abcam, Cat#15580), were applied.

Techniques: Staining

Journal: Cell Proliferation

Article Title: Dissecting the immune discrepancies in mouse liver allograft tolerance and heart/kidney allograft rejection

doi: 10.1111/cpr.13555

Figure Lengend Snippet: Ligand–receptor interactions in allografted organs. (A, B) The number of ligands and receptors involved in allografted liver (A) and the other two organs (B). (C) Ligand–receptor analysis of cytokines and immune checkpoint in the allografted liver, heart and kidney. Labels of clusters (C1: cluster1) represent the cell types in Figure (D) Representative confocal immunofluorescence images of Cd11c and Cd8 in liver allograft samples at day 7 post‐transplantation.

Article Snippet: For immunostaining, the following primary antibodies were used: rabbit anti‐mouse CD8 (Cell Signaling Technology, 98941,1:200) and Alexa Fluor 488 rat anti‐mouse CD11b (Abcam, ab197701, 1:100).

Techniques: Immunofluorescence, Transplantation Assay